PROCEDURE

       CLCuV inoculums for graft inoculation were collected from naturally infected cotton plants exhibiting characteristic leaf curl symptoms. This viral isolated, was maintained through grafting of infected stem (selected from the field) onto the plants grown in glasshouse. For artificial inoculation six weeks old plants of different varieties (table 2) were selected for graft transmission. These plants were grafted through leaf petiole graft technique, in which top of the healthy plant is removed and a wedge shaped cleft is made on the top of the stem. A diseased leaf petiole end is pointed with a sharp scalpel, is inserted as a scion and bounded with a Para film. For 24 hours kept plant at room temperature to avoid the withering of scion. Then we shifted them in glasshouse. Data of graft inoculation plants taken daily that is starting from one week after graft transmission and continued up to 90 days using 0-6 scales (table 3).

The CLCuV virus successfully transmitted through whitefly from disease to healthy plants. The transmission which involved introducing a large population of whitefly into a large muslin cage containing only infected cotton plants and leaving them for 48-72 hours. Then removed the infected plants and placed healthy plants (2^nd leaf stage) in the cage. These plants were kept up to 10 days in the cage having infected whiteflies. After 3 Days the symptoms of cotton leaf curl virus were record daily, by the same method which is discuss in table 3.